ASLAN RESEARCH GROUP

THE ASLAN RESEARCH GROUP FACILITIES AND RESOURCES
The Aslan Research Group (ARG) laboratories occupy a total of 2,900 sq. ft of laboratory space in the Mitchell Building Rooms 142 (1,500 sq. ft) (Figure 1) and 144 (1,400 sq. ft) (Figure 2). These spaces are equipped with a large fume hood, benches, solvent cabinets, upright fridge freezer, gas, air, nitrogen and vacuum lines, desks, computers, and working space for the students. The following are the equipment and research tools available for use and collaboration.


1. Microbiological and Molecular Biological Equipment

A) Human Cell Culture

1. Biosafety cabinet
2. Constant temperature water bath
3. Temperature adjustable CO2 incubator
4. Inverted microscope
5. Large-scale refrigerated centrifuge
6. Liquid nitrogen storage
7. Automated cell counter
8. Centrifuges - Micro centrifuge and large-scale centrifuge
9. UVP-Transilluminator


B) Molecular Biological Research

1. UV-Vis spectrophotometer (Varian Cary UV/Vis Bio100)
2. Temperature-controlled sample chambers
3. Varian Cary Eclipse Fluorometer with HTS and polarizer accessories
4. Orbital shaker and sonicator
5. Systems for gel electrophoresis
6. DNA Thermocycler
7. Gel documentation system
8. iCrystal system which utilizes a solid state microwave with a separate chamber that holds the iCrystal plates (PMMA platforms)
9. We have access to ASCEND Research Center equipment which include:
a. Plate reader (Apollo 11 LB 913)
b. Wes Simple Western (Protein Simple)
c. real-time PCR system
d. Cold room
10. We have access to the Flow Cytometry Service located in the Cancer Center at the University of Maryland Baltimore equipped with three analyzers: (Becton Dickinson LSR II cytometer, FACSCanto II, and FACScan) and an image cytometer (Amnis FlowSight) for sample acquisition, data analysis, and high-throughput sample acquisition.

C) Other Resources
1. Autoclave
2. Microscopes
a) Light Microscope
b) Desktop Phenom Scanning Electron Microscope
c) Florescence microscope
3. Fridges and Freezers
4. Analytical and micro-balances
5. Mixers
6. pH meter
7. Stirrers
8. Systems for agarose gel electrophoresis
9. Water purification system
10. Fume hood
11. Hotplates
12. Conductivity meters
13. Vacuum desiccators
14. Flammable and non-flammable chemical cabinets

2. Surface Chemistry Preparations
1. Oven (Model 1320)
2. RE 400 Rotary Evaporation System
3. Chemat Technologies Spin coater (model KW-4A)
4. MTI-Vacuum Controlled Atmosphere System (glove box)


3. Optical measurement Instruments
1. Agilent UV-visible Spectrophotometer
2. Varian Cary Eclipse Fluorometer with HTS and polarizer accessories
3. Ocean Optics USB 4000 High-Resolution Fluorescence Spectrophotometer equipped with an inter-changeable 1000/600-micron fiber optic system
4. Time-Correlated Single Photon-Counting instrumentation (TCSPC)
5. Laser table hosting optical components such as prisms, lenses, polarizers / beam splitters, filter wheels, doubling/tripling crystals, rotating XY stages, regular band-pass and interference filters, both absorption and reflectance neutral density filters and razor edge filters.
6. Swift Digital M10L Monocular Microscope with an integrated 5 MP digital camera. Other features include Objective Lenses: Reversed quadruple nosepiece includes DIN standard 4x, 10x, 40xr, 100xr semi-plan objectives. Total Magnification: 40x, 100x, 400x and 1000x magnification. Condenser: Includes Abbe NA 1.25 rack and pinion condenser with iris diaphragm.
7. i-RamanTM spectrometer system with high resolution combined with field-portability
8. Agilent Cary 660 FTIR, for spectrometry and research application needs


4. Commercial Simulation Software

1. COMSOL -Provides an interactive environment for modeling and simulating scientific and engineering problems.
2. MATLAB- For numerical analysis, especially algebraic and differential equations.
3. Material Studio- Allows for simulation and prediction of the interactions in materials and biomolecules.

5. High Resolution photography: Capture One 80 MP Camera with automated shutter control.

6. Battery Analysis Tools
1. Gamry Interface 1000E potentiostat
2. 8-Channel battery analyzer.

7. Customizable material printing: MakerBot 3-D Printer with duo extruder nozzles.

8. Computers, Software and Accessories
a) Aslan Research Group is equipped with 16 PCs (connected to secure network) with updated computer hardware and software and high-resolution printers for research writing and literature searches.
b) Single license and University Software: MS office, SigmaPlot, Endnote, ImageJ, FCS Express, Ideas, Norton Products and Adobe products.

Figure 1: Aslan Research Group Electronic and Optical Laboratory in Mitchell Building Room 142. PC- Personal Computer, PS- Potentiostat, BA-Battery Analyzer, HRC-High Resolution Camera, SEM- Scanning Electron Microscope, iCS- iCrystal System, BB- Bread Board, 3DP- 3-D Printer.

Figure 2: Aslan Research Group Laboratory in Mitchell Building Room 144. PC- Personal Computer, RF- Refrigerators, CC-Chemical Cabinet, FH-Fume Hood, OV-Oven, SM-Sputter Coater, IMW- Industrial Microwave, DO- Digital Oven, VAC-Vacuum Controlled Atmosphere System, PT-Potentiostat, BA-Battery Analyzer, HRC-High Resolution Camera, SEM- Scanning Electron Microscope, iCS- iCrystal System, BB- Bread Board, 3DP- 3-D Printer, CW- City Water, DI-Deionized Water, MM -Medical Microwave, LM- Light Microscopes, SP- Spectrophotometer, GL-Gas Lines, DS-Desiccator, CS-Chemicals, SN- Sonicator.