Core Lab Equipment
The Technical Staff maintains and manages equipment in good working order using manufacturer suggested QA/QC specifications, develops and uses SOPs (as living documents) for each instrument and technique, and maintains user schedules.
In addition, they will train faculty, staff, and students in proper use as per Core Laboratory SOPs, and will issue documentation and validation of said training. They will advise on proper specimen preparation for optimal results, perform assays for faculty who have limited time for experiments due to heavy teaching schedules, and coordinate instrument schedules to minimize waste of reagents and kits.
- Core Laboratory Equipment [PDF]
- Experience using the ProteinSimple Wes system: Ms. Janelle Guy, working with Dr. Kadir Aslan on a project titled "Apoptosis and innate immunity in chronic hepatitis C virus (HCV) infection," used the ProteinSimple Wes system to determine the expression of soluble TRAIL, TRAIL-R1, and TRAIL-R2 in unstimulated and stimulated peripheral blood mononuclear cells (PBMCs).
PBMCs were stimulated with lipopolysaccharide (LPS) or recombinant HCV core protein for three hours, six hours, or twelve hours. Baseline (t = 0) and unstimulated PBMCs were used as control samples. The Wes system provided a simple method to detect proteins in multiple samples within a short timeframe (compared to a conventional western blot).
According to Dr. Aslan, the Wes system has greatly contributed to the progress of the project. Wes has also been a simple method to teach other team members, helping them to understand basic concepts of immunoassay techniques. The flexibility of the digital images obtained from the Wes offer an excellent way to present data.